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PCDH11X Polyclonal Antibody, AbBy Fluor-750 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-11107R-BF750-100uL
Product Name PCDH11X Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation AbBy Fluor-750
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PCDH11X
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Cell membrane
Synonyms PCDH11; PCDH22; PCDHX; PCDHY; Protocadherin 11; Protocadherin 11 X linked; Protocadherin 11 Y linked; Protocadherin 22; Protocadherin on the X chromosome; Protocadherin on the Y chromosome; Protocadherin PC; Protocadherin prostate cancer; PC11X_HUMAN.
Background This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. The encoded protein consists of an extracellular domain containing 7 cadherin repeats, a transmembrane domain and a cytoplasmic tail that differs from those of the classical cadherins. The gene is located in a major X/Y block of homology and its Y homolog, despite divergence leading to coding region changes, is the most closely related cadherin family member. The protein is thought to play a fundamental role in cell-cell recognition essential for the segmental development and function of the central nervous system. Transcripts arising from alternative splicing encode isoforms with variable cytoplasmic domains. [provided by RefSeq, Jul 2008].
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is PCDH11X Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-11107R-BF750) validated for, and at what dilution?

bs-11107R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PCDH11X Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-11107R-BF750) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Sheep, Pig, Horse, Rabbit.

How should bs-11107R-BF750 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PCDH11X Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-11107R-BF750)?

KLH conjugated synthetic peptide derived from human PCDH11X

What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-11107R-BF750)?

AbBy Fluor-750 on bs-11107R-BF750: excitation / emission 749nm/775nm.

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