SP7/Osterix Polyclonal Antibody

Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti Osterix Polyclonal Antibody, Unconjugated (bs-1110R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Mouse spinal cord lysates probed with Osterix Polyclonal Antibody, unconjugated (bs-1110R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Mouse spinal cord lysates probed with Osterix Polyclonal Antibody, unconjugated (bs-1110R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Lane 1: Mouse Embryo tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Mouse Cerebrum tissue lysates probed with SP7\/Osterix Polyclonal Antibody, Unconjugated (bs-1110R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Embryo tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Mouse Cerebrum tissue lysates probed with SP7\/Osterix Polyclonal Antibody, Unconjugated (bs-1110R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P

Reactivity

Human
Mouse

Overview
Catalog #	bs-1110R
Product Name	SP7/Osterix Polyclonal Antibody
Applications	WB, ELISA, IHC-P
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Osterix
Immunogen Range	331-431/431
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	121340
Swiss Prot	Q8TDD2
Synonyms	OSX; OI11; OI12; osterix; Transcription factor Sp7; Zinc finger protein osterix; SP7
Background	Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences (By similarity).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400