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ROM1 Polyclonal Antibody, AbBy Fluor-350 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Cat
  • Rabbit
Overview
Catalog # bs-11125R-BF350-100uL
Product Name ROM1 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Cat, Rabbit
Specifications
Conjugation AbBy Fluor-350
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ROM1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Cell membrane
Synonyms Retinal outer segment membrane protein 1; Rod outer segment membrane protein 1; ROM; ROM1; ROM1_HUMAN; ROSP1; RP7; Tetraspanin 23; Tetraspanin-23; Tspan 23; Tspan-23; TSPAN23.
Background This gene is a member of a photoreceptor-specific gene family and encodes an integral membrane protein found in the photoreceptor disk rim of the eye. This protein can form homodimers or can heterodimerize with another photoreceptor, retinal degeneration slow (RDS). It is essential for disk morphogenesis, and may also function as an adhesion molecule involved in the stabilization and compaction of outer segment disks or in the maintenance of the curvature of the rim. Certain defects in this gene have been associated with the degenerative eye disease retinitis pigmentosa. [provided by RefSeq, Jul 2008].
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is ROM1 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-11125R-BF350) validated for, and at what dilution?

bs-11125R-BF350 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-11125R-BF350 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ROM1 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-11125R-BF350)?

KLH conjugated synthetic peptide derived from human ROM1

What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-11125R-BF350)?

AbBy Fluor-350 on bs-11125R-BF350: excitation / emission 346nm/442nm.