PVRL1 Polyclonal Antibody

$Lane 1: MCF-7 lysates probed with Rabbit Anti-Nectin1\/CD111 Polyclonal Antibody, Unconjugated (bs-11126R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.$

Lane 1: MCF-7 lysates probed with Rabbit Anti-Nectin1\/CD111 Polyclonal Antibody, Unconjugated (bs-11126R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.

$Lane 1: Mouse Urinary bladder tissue lysates; Lane 2: Rat Urinary bladder tissue lysates probed with PVRL1\/CD111 Polyclonal Antibody, Unconjugated (bs-11126R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Urinary bladder tissue lysates; Lane 2: Rat Urinary bladder tissue lysates probed with PVRL1\/CD111 Polyclonal Antibody, Unconjugated (bs-11126R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Human U87MG cell lysates; Lane 2: Human SH-SY5Y cell lysates probed with PVRL1 Polyclonal Antibody, Unconjugated (bs-11126R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human U87MG cell lysates; Lane 2: Human SH-SY5Y cell lysates probed with PVRL1 Polyclonal Antibody, Unconjugated (bs-11126R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
dot-blot

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Rabbit

Overview
Catalog #	bs-11126R
Product Name	PVRL1 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), dot-blot
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CD111/Nectin1
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5818
Swiss Prot	Q15223
Subcellular location	Secreted, Cell membrane
Synonyms	ED4; PRR; HIgR; HV1S; HVEC; OFC7; PRR1; PVRR; CD111; PVRR1; SK-12; CLPED1; nectin-1; Herpes virus entry mediator C; Herpesvirus entry mediator C; Herpesvirus Ig-like receptor; Poliovirus receptor-related protein 1; PVRL1
Background	Promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between PVRL1/nectin-1 and PVRL3/nectin-3 and between PVRL1/nectin-1 and PVRL4/nectin-4. Functions as an entry receptor for herpes simplex virus and pseudorabies virus. Has some neurite outgrowth-promoting activity.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
dot-blot