Rho B Polyclonal Antibody, PerCP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11142R-PerCP-100uL |
| Product Name | Rho B Polyclonal Antibody, PerCP Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Chicken, Rabbit |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Rho B |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 388 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | Rh -related GTP binding protein RhoB; Rh -related GTP binding protein RhoC; RHOH6; Transforming protein RhoA; AA017882 antibody Aplysia ras related homolog 9 RhoC; ARH9; ARHA; ARHB; ARHC; H6; MST081; MSTP08; Oncogene RHO H9; rhoC GTPase; Ras homolog 9 RhoC; Ras homolog gene family, member A; Ras homolog gene family, member B; Ras homolog gene family, member C; Rho related GTP binding protein RhoC; RHO12; RHOB; RHOC; RHOH9; RP11-426L16.4; Small GTP binding protein RhoC; H9; Rho related GTP binding protein RhoB; ARH12; ARH6; H12.RHOB_HUMAN |
| Background | The Rho proteins interact directly with protein kinases which are likely to serve as downstream effector targets of the activated GTPase. The Rho family of GTPases are involved in the regulation of the actin skeleton. Activation of Rho to the GTP bound state promotes formation of stress fibers and is required for the formation and maintenance of focal adhesions. Pathways stimulated by cytokines, hormones and various forms of stress utilize p21 proteins of the Rho family. Possible mediators for these responses include protein kinase such as p120PKN, and p150 ROKa/Rho-A kinase, myosin light chain (MLC) phosphatase and PIP 5-kinase. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Rho B Polyclonal Antibody, PerCP Conjugated (bs-11142R-PerCP) validated for, and at what dilution?
bs-11142R-PerCP is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Rho B Polyclonal Antibody, PerCP Conjugated (bs-11142R-PerCP) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Chicken, Rabbit.
How should bs-11142R-PerCP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Rho B Polyclonal Antibody, PerCP Conjugated (bs-11142R-PerCP)?
KLH conjugated synthetic peptide derived from human Rho B
What are the excitation and emission wavelengths of the PerCP conjugate (bs-11142R-PerCP)?
PerCP on bs-11142R-PerCP: excitation / emission 482nm/677nm.