PCDHGC3 Polyclonal Antibody, APC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11159R-APC |
| Product Name | PCDHGC3 Polyclonal Antibody, APC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Horse |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PCDHGC3/PCDH2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cell membrane |
| Synonyms | Cadherin like 2; PC 43; PC43; PCDH 2; PCDH gamma C3; PCDH2; PCDHGC 3; Protocadherin 2; Protocadherin 43; Protocadherin gamma C3; Protocadherin gamma subfamily C 3; Protocadherin43; PCDGK_HUMAN. |
| Background | Protocadherins are a large family of cadherin-like cell adhesion proteins that are involved in the establishment and maintenance of neuronal connections in the brain. There are three protocadherin gene clusters, designated alpha, beta and gamma, all of which contain multiple tandemly arranged genes. PCDH2 (protocadherin-2), also known as PCDHGC3 (protocadherin gamma subfamily C, 3) or PC43, is a 934 amino acid single-pass type I membrane protein that contains six cadherin domains and belongs to the protocadherin gamma family. Functioning as a calcium-dependent cell-adhesion protein, PCDH2 is thought to be involved in the establishment and maintenance of neuronal connections within the brain. Multiple isoforms of PCDH2 exist due to alternative splicing events. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PCDHGC3 Polyclonal Antibody, APC Conjugated (bs-11159R-APC) validated for, and at what dilution?
bs-11159R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PCDHGC3 Polyclonal Antibody, APC Conjugated (bs-11159R-APC) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Horse.
How should bs-11159R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PCDHGC3 Polyclonal Antibody, APC Conjugated (bs-11159R-APC)?
KLH conjugated synthetic peptide derived from human PCDHGC3/PCDH2
What are the excitation and emission wavelengths of the APC conjugate (bs-11159R-APC)?
APC on bs-11159R-APC: excitation / emission 650nm/660nm.