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PCDHGC3 Polyclonal Antibody, AbBy Fluor-405 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Horse
Overview
Catalog # bs-11159R-BF405
Product Name PCDHGC3 Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Horse
Specifications
Conjugation AbBy Fluor-405
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PCDHGC3/PCDH2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Cell membrane
Synonyms Cadherin like 2; PC 43; PC43; PCDH 2; PCDH gamma C3; PCDH2; PCDHGC 3; Protocadherin 2; Protocadherin 43; Protocadherin gamma C3; Protocadherin gamma subfamily C 3; Protocadherin43; PCDGK_HUMAN.
Background Protocadherins are a large family of cadherin-like cell adhesion proteins that are involved in the establishment and maintenance of neuronal connections in the brain. There are three protocadherin gene clusters, designated alpha, beta and gamma, all of which contain multiple tandemly arranged genes. PCDH2 (protocadherin-2), also known as PCDHGC3 (protocadherin gamma subfamily C, 3) or PC43, is a 934 amino acid single-pass type I membrane protein that contains six cadherin domains and belongs to the protocadherin gamma family. Functioning as a calcium-dependent cell-adhesion protein, PCDH2 is thought to be involved in the establishment and maintenance of neuronal connections within the brain. Multiple isoforms of PCDH2 exist due to alternative splicing events.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is PCDHGC3 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-11159R-BF405) validated for, and at what dilution?

bs-11159R-BF405 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PCDHGC3 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-11159R-BF405) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Horse.

How should bs-11159R-BF405 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PCDHGC3 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-11159R-BF405)?

KLH conjugated synthetic peptide derived from human PCDHGC3/PCDH2

What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-11159R-BF405)?

AbBy Fluor-405 on bs-11159R-BF405: excitation / emission 401nm/421nm.