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PCDHGC3 Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Horse
Overview
Catalog # bs-11159R-PE
Product Name PCDHGC3 Polyclonal Antibody, PE Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Horse
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PCDHGC3/PCDH2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Cell membrane
Synonyms Cadherin like 2; PC 43; PC43; PCDH 2; PCDH gamma C3; PCDH2; PCDHGC 3; Protocadherin 2; Protocadherin 43; Protocadherin gamma C3; Protocadherin gamma subfamily C 3; Protocadherin43; PCDGK_HUMAN.
Background Protocadherins are a large family of cadherin-like cell adhesion proteins that are involved in the establishment and maintenance of neuronal connections in the brain. There are three protocadherin gene clusters, designated alpha, beta and gamma, all of which contain multiple tandemly arranged genes. PCDH2 (protocadherin-2), also known as PCDHGC3 (protocadherin gamma subfamily C, 3) or PC43, is a 934 amino acid single-pass type I membrane protein that contains six cadherin domains and belongs to the protocadherin gamma family. Functioning as a calcium-dependent cell-adhesion protein, PCDH2 is thought to be involved in the establishment and maintenance of neuronal connections within the brain. Multiple isoforms of PCDH2 exist due to alternative splicing events.
Application Dilution
WB 1:300-5000
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Frequently asked questions

Which applications is PCDHGC3 Polyclonal Antibody, PE Conjugated (bs-11159R-PE) validated for, and at what dilution?

bs-11159R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PCDHGC3 Polyclonal Antibody, PE Conjugated (bs-11159R-PE) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Horse.

How should bs-11159R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PCDHGC3 Polyclonal Antibody, PE Conjugated (bs-11159R-PE)?

KLH conjugated synthetic peptide derived from human PCDHGC3/PCDH2

What are the excitation and emission wavelengths of the PE conjugate (bs-11159R-PE)?

PE on bs-11159R-PE: excitation / emission 496,564nm/578nm.