VGLUT1/BNP1 Polyclonal Antibody

$Antigen: bs-11167P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450$

Antigen: bs-11167P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450

Mouse brain lysates probed with Rabbit Anti-VGLUT1 Polyclonal Antibody, Unconjugated (bs-11167R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody for 90 min at 37˚C.

$Lane 1: Rat Cerebrum tissue lysates; Lane 2: Human SH-SY5Y cell lysates; Lane 3: Human MCF-7 cell lysates probed with VGLUT1\/BNP1 Polyclonal Antibody, Unconjugated (bs-11167R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Rat Cerebrum tissue lysates; Lane 2: Human SH-SY5Y cell lysates; Lane 3: Human MCF-7 cell lysates probed with VGLUT1\/BNP1 Polyclonal Antibody, Unconjugated (bs-11167R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VGLUT1\/BNP1 Polyclonal Antibody, Unconjugated (bs-11167R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VGLUT1\/BNP1 Polyclonal Antibody, Unconjugated (bs-11167R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VGLUT1\/BNP1 Polyclonal Antibody, Unconjugated (bs-11167R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VGLUT1\/BNP1 Polyclonal Antibody, Unconjugated (bs-11167R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VGLUT1\/BNP1 Polyclonal Antibody, Unconjugated (bs-11167R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VGLUT1\/BNP1 Polyclonal Antibody, Unconjugated (bs-11167R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Mouse
Rat

Predicted Reactivity

Human

Overview
Catalog #	bs-11167r
Product Name	VGLUT1/BNP1 Polyclonal Antibody
Applications	WB, IHC-P, IHC-F, IF
Reactivity	Mouse, Rat
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human VGLUT1/BNP1
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	57030
Swiss Prot	Q9P2U7
Subcellular location	Cytoplasm, Cell membrane
Synonyms	BNPI; Brain specific Na(+) dependent inorganic phosphate cotransporter; Brain specific Na dependent inorganic phosphate cotransporter; Brain-specific Na(+)-dependent inorganic phosphate cotransporter; Slc17a7; Solute carrier family 17(sodium-dependent inorganic phosphate cotransporter), member 7; Solute carrier family 17 member 7; Vesicular glutamate transporter 1; VGLU1_HUMAN; VGluT1.
Application Dilution
WB	=1:500-1000
IHC-P	IHC-P=1:100-500
IHC-F	IHC-F=1:100-500
IF	IF=1:100-500