NAP1L2 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11172R-BF350-100uL |
| Product Name | NAP1L2 Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NAP1L2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Nucleus |
| Synonyms | Brain specic gene BPX; BPX; Brain specic protein, X linked; MGC26243; Nucleosome assembly protein 1 like 2; NP1L2_HUMAN. |
| Background | Proper nucleosome assembly is critical for compacting DNA into chromatin. In human and mouse there are 5 protein-coding genes which comprise the nucleosome assembly protein (NAP) family. NAP1L1 (NAP1) and NAP1L4 (NAP2) are ubiquitously expressed family members which have been the most extensively studied. The remaining three family members, NAP1L2, NAP1L3 and NAP1L5 are neuron-specific nucleosome assembly proteins translated from intronless genes which are monoallelically expressed. NAP1L2 (nucleosome assembly protein 1-like 2), also known as BPX (brain specific protein, X-linked), is a 460 amino acid protein containing acidic domains which are thought to mediate histone interactions. NAP1L2 binds to chromatin and interacts with Histones H3 and H4. The function of NAP1L2 is not clearly defined although evidence suggests that NAP1L2 influences histone acetylation and therefore may play a significant role in regulating transcription in developing neurons. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NAP1L2 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-11172R-BF350) validated for, and at what dilution?
bs-11172R-BF350 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NAP1L2 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-11172R-BF350) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Pig, Horse, Rabbit.
How should bs-11172R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NAP1L2 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-11172R-BF350)?
KLH conjugated synthetic peptide derived from human NAP1L2
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-11172R-BF350)?
AbBy Fluor-350 on bs-11172R-BF350: excitation / emission 346nm/442nm.