Signal Peptide Peptidase Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-11178R-BF750-100uL |
| Product Name | Signal Peptide Peptidase Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Signal Peptide Peptidase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 207 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Histocompatibility minor 13; HM 13; HM13; HM13_HUMAN; IMP 1; IMP-1; IMP1; IMPAS; IMPAS-1; Intramembrane Protease 1; Intramembrane protease; Minor Histocompatibility 13; Minor histocompatibility antigen 13; Minor histocompatibility antigen H13; MSTP086; Presenilin like protein 3; Presenilin-like protein 3; PSENL 3; PSENL3; PSL 3; PSL3; Signal peptide peptidase; Signal peptide peptidase beta; SPP; dJ324O17.1; H13; hIMP1; hIMP1 protein. |
| Background | The endoplasmic reticulum exerts a quality control over newly synthesized proteins and a variety of components have been implicated in the specific recognition of aberrant or misfolded polypeptides. Signal peptide peptidase (SPP) catalyzes intramembrane proteolysis of some signal peptides after they have been cleaved from a preprotein, resulting in the release of the fragment from the ER membrane into the cytoplasm. SPP is required to generate lymphocyte cell surface (HLA-E) epitopes derived from MHC class I signal peptides, and may play a role in graft rejection. It also may be necessary for the removal of the signal peptide that remains attached to the hepatitis C virus core protein after the initial proteolytic processing of the polyprotein. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Signal Peptide Peptidase Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-11178R-BF750) validated for, and at what dilution?
bs-11178R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-11178R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Signal Peptide Peptidase Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-11178R-BF750)?
KLH conjugated synthetic peptide derived from human Signal Peptide Peptidase
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-11178R-BF750)?
AbBy Fluor-750 on bs-11178R-BF750: excitation / emission 749nm/775nm.