MAdCAM1 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-11179R-BF680-100uL |
| Product Name | MAdCAM1 Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MAdCAM1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8174 |
| Swiss Prot | Q13477 |
| Subcellular location | Cell membrane |
| Synonyms | MACAM1; Mucosal addressin cell adhesion molecule 1; MAdCAM-1; hMAdCAM-1; MADCAM1 |
| Background | Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is MAdCAM1 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-11179R-BF680) validated for, and at what dilution?
bs-11179R-BF680 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-11179R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MAdCAM1 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-11179R-BF680)?
KLH conjugated synthetic peptide derived from human MAdCAM1
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-11179R-BF680)?
AbBy Fluor-680 on bs-11179R-BF680: excitation / emission 679nm/702nm.