Complement component C9a Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-11190R-Cy5 |
| Product Name | Complement component C9a Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Complement component C9a |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Secreted, Cell membrane |
| Synonyms | C9a, C9 deficiency; C9 deficiency with dermatomyositis; CO9_HUMAN; Complement component 9; Complement component 9 deficiency; Complement component C9a; Complement component C9a; C9. |
| Background | C9 is a plasma protein synthesized in the liver and monocytes consisting of a single polypeptide chain. C9 is a part of the membrane attack complex (MAC), an important component of the immune system. The MAC forms upon complement system activation by invading pathogenic bacteria and consists of the four major complement proteins: C5b, C6, C7 and C8. These complement proteins bind to the outer surface of the plasma membrane of the invading cell. C9 binds to the membrane associated C5b-8 protein, which leads to the circular polymerization of 12-18 C9 molecules. These polymerized C9 molecules form a ring structure in the membrane. Molecules can then diffuse freely through this transmembrane channel, causing cell lysis and destruction of the invading bacterial cell. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Complement component C9a Polyclonal Antibody, Cy5 Conjugated (bs-11190R-Cy5) validated for, and at what dilution?
bs-11190R-Cy5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Complement component C9a Polyclonal Antibody, Cy5 Conjugated (bs-11190R-Cy5) react with?
Tested reactivity: Human.
How should bs-11190R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Complement component C9a Polyclonal Antibody, Cy5 Conjugated (bs-11190R-Cy5)?
KLH conjugated synthetic peptide derived from human Complement component C9a
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-11190R-Cy5)?
Cy5 on bs-11190R-Cy5: excitation / emission 625,650nm/670nm.