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Musashi 1 Polyclonal Antibody

Formalin-fixed and paraffin embedded mouse brain labeled with Anti-Musashi 1\/Msi1 Polyclonal Antibody, Unconjugated (bs-11201R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Formalin-fixed and paraffin embedded mouse brain labeled with Anti-Musashi 1\/Msi1 Polyclonal Antibody, Unconjugated (bs-11201R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-Musashi 1\/Msi1 Polyclonal Antibody, Unconjugated (bs-11201R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-Musashi 1\/Msi1 Polyclonal Antibody, Unconjugated (bs-11201R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Musashi 1\/2 Polyclonal Antibody, Unconjugated (bs-11201R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Musashi 1\/2 Polyclonal Antibody, Unconjugated (bs-11201R) at 1:200 overnight at 4\u00b0C, DAB staining.
SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Musashi 1) polyclonal Antibody, Unconjugated (bs-11201R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Musashi 1) polyclonal Antibody, Unconjugated (bs-11201R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
SH-SY5Y cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Musashi 1 Antibody(bs-11201R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
SH-SY5Y cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Musashi 1 Antibody(bs-11201R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
Bioss unconjugated antibody vial

Applications

  • IHC-P
  • IHC-F
  • IF
  • Flow-Cyt
  • ICC/IF

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Pig
  • Chicken
  • Rabbit
Overview
Catalog # bs-11201R
Product Name Musashi 1 Polyclonal Antibody
Applications
IHC-P IHC-F IF FLOW-CYT ICC/IF
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Pig, Chicken, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Musashi 1
Immunogen Range 66-150/362
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 4440, 124540
Subcellular location Cytoplasm, Nucleus
Synonyms Msi 1; Msi1; Msi-1; MSI1H_HUMAN; Musashi homolog 1; Musashi-1; Musashi1; Musashi 1; RNA binding protein Musashi homolog 1; RNA-binding protein Musashi homolog 1; Msi 2; Msi2; Msi-2; MSI2H_HUMAN; Musashi homolog 2; Musashi-2; Musashi2; Musashi 2; RNA binding protein Musashi homolog 2; RNA-binding protein Musashi homolog 2.
Background Musashi1 (Msi1) is an RNA-binding protein expressed in neural progenitor cells and neural stem cells. Msi1 is the mammalian homolog of Drosophila Musashi. The gene encoding human Msi1 encodes a 362 amino acid protein. In murine embryonic neural progenitor cells, Msi1 localizes to the cytoplasm and is downregulated during differentiation. Msi1 binds to NUMB, which encodes a membrane-associated antagonist of Notch signaling. Msi1 appears to function in the proliferation and maintenance of stem cell populations of the central nervous system. In addition to its usefulness as a marker for neural progenitor cells in normal human brains, Msi1 is also a marker for human gliomas. In rats, Msi1 is expressed in Sertoli cells of the testis and granulosa cells of the ovary.
Application Dilution
IHC-P 1:200-400
IHC-F 1:100-500
IF IHC-P1:50-200
Flow-Cyt IFIHC-F1:50-200
ICC/IF IFICC1:50-200

Frequently asked questions

Which applications is Musashi 1 Polyclonal Antibody (bs-11201R) validated for, and at what dilution?

bs-11201R is validated for IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Musashi 1 Polyclonal Antibody (bs-11201R) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Pig, Chicken, Rabbit.

How should bs-11201R be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Musashi 1 Polyclonal Antibody (bs-11201R)?

KLH conjugated synthetic peptide derived from human Musashi 1 Immunogen range: 66-150/362.

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