Musashi 1 Polyclonal Antibody

$Formalin-fixed and paraffin embedded mouse brain labeled with Anti-Musashi 1\/Msi1 Polyclonal Antibody, Unconjugated (bs-11201R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n$

Formalin-fixed and paraffin embedded mouse brain labeled with Anti-Musashi 1\/Msi1 Polyclonal Antibody, Unconjugated (bs-11201R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

$Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-Musashi 1\/Msi1 Polyclonal Antibody, Unconjugated (bs-11201R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n$

Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-Musashi 1\/Msi1 Polyclonal Antibody, Unconjugated (bs-11201R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

$Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Musashi 1\/2 Polyclonal Antibody, Unconjugated (bs-11201R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Musashi 1\/2 Polyclonal Antibody, Unconjugated (bs-11201R) at 1:200 overnight at 4\u00b0C, DAB staining.

$SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Musashi 1) polyclonal Antibody, Unconjugated (bs-11201R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Musashi 1) polyclonal Antibody, Unconjugated (bs-11201R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

$SH-SY5Y cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Musashi 1 Antibody(bs-11201R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).$

SH-SY5Y cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Musashi 1 Antibody(bs-11201R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Applications

IHC-P
IHC-F
IF
Flow-Cyt
ICC/IF

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Dog
Cow
Pig
Chicken
Rabbit

Overview
Catalog #	bs-11201R
Product Name	Musashi 1 Polyclonal Antibody
Applications	IHC-P IHC-F IF FLOW-CYT ICC/IF
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Dog, Cow, Pig, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Musashi 1
Immunogen Range	66-150/362
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4440, 124540
Subcellular location	Cytoplasm, Nucleus
Synonyms	Msi 1; Msi1; Msi-1; MSI1H_HUMAN; Musashi homolog 1; Musashi-1; Musashi1; Musashi 1; RNA binding protein Musashi homolog 1; RNA-binding protein Musashi homolog 1; Msi 2; Msi2; Msi-2; MSI2H_HUMAN; Musashi homolog 2; Musashi-2; Musashi2; Musashi 2; RNA binding protein Musashi homolog 2; RNA-binding protein Musashi homolog 2.
Background	Musashi1 (Msi1) is an RNA-binding protein expressed in neural progenitor cells and neural stem cells. Msi1 is the mammalian homolog of Drosophila Musashi. The gene encoding human Msi1 encodes a 362 amino acid protein. In murine embryonic neural progenitor cells, Msi1 localizes to the cytoplasm and is downregulated during differentiation. Msi1 binds to NUMB, which encodes a membrane-associated antagonist of Notch signaling. Msi1 appears to function in the proliferation and maintenance of stem cell populations of the central nervous system. In addition to its usefulness as a marker for neural progenitor cells in normal human brains, Msi1 is also a marker for human gliomas. In rats, Msi1 is expressed in Sertoli cells of the testis and granulosa cells of the ovary.
Application Dilution
IHC-P	1:200-400
IHC-F	1:100-500
IF	IHC-P1:50-200
Flow-Cyt	IFIHC-F1:50-200
ICC/IF	IFICC1:50-200