TCF12 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11237R-Cy5.5-100uL |
| Product Name | TCF12 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Sheep, Horse, Rabbit, Zebrafish |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TCF12 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6938 |
| Swiss Prot | Q99081 |
| Subcellular location | Nucleus |
| Synonyms | bHLHb20; Class B basic helix-loop-helix protein 20; DNA binding protein HTF4; DNA-binding protein HTF4; E box binding protein; E-box-binding protein; HEB; HTF4; HTF4_HUMAN; TCF-12; Tcf12; Transcription factor 12; Transcription factor HTF-4; Transcription factor HTF4. |
| Background | Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development includes Myo D, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). Myo D transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. Myo D-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes. Both homo- and hetero-oligomers of these proteins are able to distinguish very closely related E box proteins and are believed to play important roles in lineage specific gene expression. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is TCF12 Polyclonal Antibody, Cy5.5 Conjugated (BS-11237R-CY5-5) validated for, and at what dilution?
BS-11237R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TCF12 Polyclonal Antibody, Cy5.5 Conjugated (BS-11237R-CY5-5) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Sheep, Horse, Rabbit, Zebrafish.
How should BS-11237R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TCF12 Polyclonal Antibody, Cy5.5 Conjugated (BS-11237R-CY5-5)?
KLH conjugated synthetic peptide derived from human TCF12
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-11237R-CY5-5)?
Cy5.5 on BS-11237R-CY5-5: excitation / emission 675nm/694nm.