Flotillin 2 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11254R-RBITC-100uL |
| Product Name | Flotillin 2 Polyclonal Antibody, RBITC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ECS1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2319 |
| Swiss Prot | Q14254 |
| Subcellular location | Cell membrane |
| Synonyms | ESA; ECS1; ESA1; ECS-1; M17S1; Flotillin-2; Epidermal surface antigen; Membrane component chromosome 17 surface marker 1; FLOT2 |
| Background | May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Flotillin 2 Polyclonal Antibody, RBITC Conjugated (bs-11254R-RBITC) validated for, and at what dilution?
bs-11254R-RBITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Flotillin 2 Polyclonal Antibody, RBITC Conjugated (bs-11254R-RBITC) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig, Horse.
How should bs-11254R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Flotillin 2 Polyclonal Antibody, RBITC Conjugated (bs-11254R-RBITC)?
KLH conjugated synthetic peptide derived from human ECS1
What are the excitation and emission wavelengths of the RBITC conjugate (bs-11254R-RBITC)?
RBITC on bs-11254R-RBITC: excitation / emission 540nm/625nm.