SNIP1 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-11260R-BF680-100uL |
| Product Name | SNIP1 Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SNIP1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Nucleus |
| Synonyms | FHA domain-containing protein SNIP1; FLJ12553; Smad nuclear interacting protein Smad nuclear interacting; Smad nuclear interacting protein; Smad nuclear-interacting protein 1; SNIP1 Smad nuclear interacting protein; SNIP1; SNIP1_HUMAN; Splicing factor arginine/serine rich 4 Pre mRNA splicing factor SRP75. |
| Background | Members of the transforming growth factor-beta (TGF-Beta) superfamily play critical roles in controlling cell growth and differentiation. Effects of TGF-Beta family ligands are mediated by Smad proteins. The Smad nuclear interacting protein (SNIP1) contains a forkhead-associated (FHA) domain and acts as a nuclear inhibitor of CBP/p300. SNIP1 potently inhibits the activity of NF-kappa B, which binds the C/H1 domain of CBP/p300, by competing for the binding site. SNIP1 is also thought to induce expression of Cyclin D1 to promote cellular proliferation. SNIP1 is ubiquitously expressed with high expression in heart and skeletal muscle. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is SNIP1 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-11260R-BF680) validated for, and at what dilution?
bs-11260R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-11260R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SNIP1 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-11260R-BF680)?
KLH conjugated synthetic peptide derived from human SNIP1
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-11260R-BF680)?
AbBy Fluor-680 on bs-11260R-BF680: excitation / emission 679nm/702nm.