phospho-CTBP1 (Ser422) Polyclonal Antibody, PE Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11275R-PE-100uL |
| Product Name | phospho-CTBP1 (Ser422) Polyclonal Antibody, PE Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Horse, Chicken, Zebrafish |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human CTBP1 around the phosphorylation site of Ser422 |
| Modification Site | Ser422 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | CTBP1 phospho Ser422; p-CTBP1 Ser422; CtBP1 phospho S422; BARS; C terminal binding protein 1; CTBP; CTBP1; MGC104684; CTBP1_HUMAN; C-terminal-binding protein 1; CtBP1. |
| Background | CtBP1 associates in complexes with histone deacetylases (HDAC), the G9a histone methyltransferase, and LSD1 histone demethylase illustrating its role in transcriptional repression. Through its association with centrosomes, CtBP1 appears to also play a role in mitosis and Golgi complex organization. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-CTBP1 (Ser422) Polyclonal Antibody, PE Conjugated (bs-11275R-PE) validated for, and at what dilution?
bs-11275R-PE is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-CTBP1 (Ser422) Polyclonal Antibody, PE Conjugated (bs-11275R-PE) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Horse, Chicken, Zebrafish.
How should bs-11275R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-CTBP1 (Ser422) Polyclonal Antibody, PE Conjugated (bs-11275R-PE)?
KLH conjugated synthetic phosphopeptide derived from human CTBP1 around the phosphorylation site of Ser422
What are the excitation and emission wavelengths of the PE conjugate (bs-11275R-PE)?
PE on bs-11275R-PE: excitation / emission 496,564nm/578nm.