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CTBP1 (Ser422) Polyclonal Antibody, PerCP-Cy7 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Dog
  • Cow
  • Horse
  • Chicken
  • Zebrafish
Overview
Catalog # bs-11275r-percp-cy7-100uL
Product Name CTBP1 (Ser422) Polyclonal Antibody, PerCP-Cy7 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Rat
Predicted Reactivity Human, Mouse, Dog, Cow, Horse, Chicken, Zebrafish
Specifications
Conjugation PerCP-Cy7
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human CTBP1 around the phosphorylation site of Ser422
Modification Site Ser422
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Cytoplasm, Nucleus
Synonyms CTBP1 phospho Ser422; p-CTBP1 Ser422; CtBP1 phospho S422; BARS; C terminal binding protein 1; CTBP; CTBP1; MGC104684; CTBP1_HUMAN; C-terminal-binding protein 1; CtBP1.
Background CtBP1 associates in complexes with histone deacetylases (HDAC), the G9a histone methyltransferase, and LSD1 histone demethylase illustrating its role in transcriptional repression. Through its association with centrosomes, CtBP1 appears to also play a role in mitosis and Golgi complex organization.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CTBP1 (Ser422) Polyclonal Antibody, PerCP-Cy7 Conjugated (bs-11275R-PerCP-Cy7) validated for, and at what dilution?

bs-11275R-PerCP-Cy7 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CTBP1 (Ser422) Polyclonal Antibody, PerCP-Cy7 Conjugated (bs-11275R-PerCP-Cy7) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Horse, Chicken, Zebrafish.

How should bs-11275R-PerCP-Cy7 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CTBP1 (Ser422) Polyclonal Antibody, PerCP-Cy7 Conjugated (bs-11275R-PerCP-Cy7)?

KLH conjugated synthetic phosphopeptide derived from human CTBP1 around the phosphorylation site of Ser422

What are the excitation and emission wavelengths of the PerCP-Cy7 conjugate (bs-11275R-PerCP-Cy7)?

PerCP-Cy7 on bs-11275R-PerCP-Cy7: excitation / emission 482nm/780nm.