ESE1 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11279R-Cy5.5-100uL |
| Product Name | ESE1 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ESE1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | E74 like factor 3; E74 like factor 3 ets domain transcription factor; E74 like factor 3 ets domain transcription factor epithelial specic; E74 like factor 3 ETS domain transcription factor serine box epithelial specic; E74-like factor 3; Elf3; ELF3_HUMAN; Epithelial restricted with serine box; Epithelial-restricted with serine box; Epithelium restricted Ets protein ESX; Epithelium specic Ets factor 1; Epithelium specic Ets transcription factor 1; Epithelium-restricted Ets protein ESX; Epithelium-specic Ets transcription factor 1; EPR 1; EPR1; ERT; ESE-1; ESX; Ets domain transcription factor serine box; Ets domain transcription factor serine box epithelial specic; Ets transcription factor; ETS-related transcription factor Elf-3; jen; MGC139318. |
| Background | ESE-1, a member of the Ets family of transcription factors, critically regulates epithelial cell differentiation and mediates vascular inflammation. ESE-1 is strongly expressed in vascular endothelium and smooth muscle cells where it is induced in response to inflammatory cytokines and lipopolysaccharides, interacts with NF-KappaB to induce nitric oxide synthase, and is induced during terminal differentiation of epidermal and primary keratinocytes. In addition, ESE-1 is upregulated upon differentiation of corneal epithelium and interacts with Sp1 and AP-1 proteins to induce squamous differentiation marker expression in bronchial epithelial cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ESE1 Polyclonal Antibody, Cy5.5 Conjugated (BS-11279R-CY5-5) validated for, and at what dilution?
BS-11279R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ESE1 Polyclonal Antibody, Cy5.5 Conjugated (BS-11279R-CY5-5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep.
How should BS-11279R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ESE1 Polyclonal Antibody, Cy5.5 Conjugated (BS-11279R-CY5-5)?
KLH conjugated synthetic peptide derived from human ESE1
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-11279R-CY5-5)?
Cy5.5 on BS-11279R-CY5-5: excitation / emission 675nm/694nm.