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FABP3/H-FABP Polyclonal Antibody

Applications

  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-11283R-100uL
Product Name FABP3/H-FABP Polyclonal Antibody
Applications
ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity This antibody will recognize FABP in heart and has a high sequence similarity with FABP found in adipocytes.
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human FABP3
Immunogen Range 8-100/133
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 2170
Swiss Prot P05413
Subcellular location Cytoplasm
Synonyms MDGI; FABP11; H-FABP; M-FABP; O-FABP; Fatty acid-binding protein, heart; Fatty acid-binding protein 3; Heart-type fatty acid-binding protein; Mammary-derived growth inhibitor; Muscle fatty acid-binding protein; FABP3
Background Fatty acid-binding proteins, designated FABPs, are a family of homologous cytoplasmic proteins that are expressed in a highly tissue-specific manner and play an integral role in the balance between lipid and carbohydrate metabolism. FABPs mediate fatty acid (FA) and/or hydrophobic ligand uptake, transport and targeting within their respective tissues. The mechanisms underlying these actions can give rise to both passive diffusional uptake and protein-mediated transmembrane transport of FAs. FABPs are expressed in adipocytes (A-FABP), brain (B-FABP), epidermis (E-FABP, also designated psoriasis-associated FABP or PA-FABP), muscle and heart (H-FABP, also designated mammary-derived growth inhibitor or MDGI), intestine (I-FABP), liver (L-FABP), myelin (M-FABP) and testis (T-FABP). MDGI is highly expressed in the myocardium, skeletal and smooth muscle fibers, lipid and/or steroid synthesizing cells and terminally differentiated epithelia of the respiratory, intestinal and urogenital tracts.
Application Dilution
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is FABP3/H-FABP Polyclonal Antibody (bs-11283R) validated for, and at what dilution?

bs-11283R is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does FABP3/H-FABP Polyclonal Antibody (bs-11283R) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig, Horse, Rabbit.

How should bs-11283R be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise FABP3/H-FABP Polyclonal Antibody (bs-11283R)?

KLH conjugated synthetic peptide derived from human FABP3 Immunogen range: 8-100/133.

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