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FABP3/H-FABP Polyclonal Antibody, AbBy Fluor-405 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-11283R-BF405-100uL
Product Name FABP3/H-FABP Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity This antibody will recognize FABP in heart and has a high sequence similarity with FABP found in adipocytes.
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation AbBy Fluor-405
Host Rabbit
Source KLH conjugated synthetic peptide derived from human FABP3
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2170
Swiss Prot P05413
Subcellular location Cytoplasm
Synonyms MDGI; FABP11; H-FABP; M-FABP; O-FABP; Fatty acid-binding protein, heart; Fatty acid-binding protein 3; Heart-type fatty acid-binding protein; Mammary-derived growth inhibitor; Muscle fatty acid-binding protein; FABP3
Background Fatty acid-binding proteins, designated FABPs, are a family of homologous cytoplasmic proteins that are expressed in a highly tissue-specific manner and play an integral role in the balance between lipid and carbohydrate metabolism. FABPs mediate fatty acid (FA) and/or hydrophobic ligand uptake, transport and targeting within their respective tissues. The mechanisms underlying these actions can give rise to both passive diffusional uptake and protein-mediated transmembrane transport of FAs. FABPs are expressed in adipocytes (A-FABP), brain (B-FABP), epidermis (E-FABP, also designated psoriasis-associated FABP or PA-FABP), muscle and heart (H-FABP, also designated mammary-derived growth inhibitor or MDGI), intestine (I-FABP), liver (L-FABP), myelin (M-FABP) and testis (T-FABP). MDGI is highly expressed in the myocardium, skeletal and smooth muscle fibers, lipid and/or steroid synthesizing cells and terminally differentiated epithelia of the respiratory, intestinal and urogenital tracts.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is FABP3/H-FABP Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-11283R-BF405) validated for, and at what dilution?

bs-11283R-BF405 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does FABP3/H-FABP Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-11283R-BF405) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig, Horse, Rabbit.

How should bs-11283R-BF405 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise FABP3/H-FABP Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-11283R-BF405)?

KLH conjugated synthetic peptide derived from human FABP3

What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-11283R-BF405)?

AbBy Fluor-405 on bs-11283R-BF405: excitation / emission 401nm/421nm.

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