Lymphotactin Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-11287R-BF594-100uL |
| Product Name | Lymphotactin Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Lymphotactin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Secreted |
| Synonyms | Lptn; LTN; XCL1 C mot chemokine 1; SCM 1 alpha; ATAC; Chemokine C Mot Ligand 1; Cytokine SCM-1; LPTN; LTN; Lymphotactin; Lymphotaxin; SCM-1-alpha; SCM1; SCYC1; Small inducible cytokine C1; Small Inducible Cytokine Subfamily C Member 1; Small-inducible cytokine C1; XC chemokine ligand 1; Xcl1; XCL1_HUMAN. |
| Background | Lymphotactin is a chemokine-like molecule produced selectively, if not exclusively, by activated CD8+ T cells (1). Lymphotactin is a C-type member of the chemokine superfamily, but retains only the 2nd and 4th of the four cysteine residues conserved in other chemokines (3,4). In normal tissues, lymphotactin is expressed at high levels in spleen, thymus, small intestine, and peripheral blood leukocytes, as well as at low levels in lung, prostate, and ovary (2). Lymphotactin is chemotactic for CD4+ and CD8+ T cells but not for monocytes, and induces a rise in intracellular calcium in peripheral blood lymphocytes (1). The specific receptor for lymphotactin is XCR1, a member of the G protein-coupled receptor family (5). The gene which encodes lymphotactin maps to human chromosome 1q23 (1,2). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Lymphotactin Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-11287R-BF594) validated for, and at what dilution?
bs-11287R-BF594 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-11287R-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Lymphotactin Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-11287R-BF594)?
KLH conjugated synthetic peptide derived from human Lymphotactin
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-11287R-BF594)?
AbBy Fluor-594 on bs-11287R-BF594: excitation / emission 590nm/617nm.