NAB2 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11299R-BF680-100uL |
| Product Name | NAB2 Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NAB2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Nucleus |
| Synonyms | EGR 1 binding protein 2; EGR-1-binding protein 2; EGR1 binding protein 2; MADER; Melanoma associated delayed early response protein; Melanoma-associated delayed early response protein; MGC75085; Nab 2; nab2; NAB2_HUMAN; NGFI A binding protein 2 EGR1 binding protein 2; NGFI A binding protein 2; NGFI-A-binding protein 2; Protein MADER. |
| Background | Transcriptional control is in part regulated by interactions between DNA-bound transcription factors, such as Egr-1/NGFI-A, and coregulatory proteins, such as NAB (for NGFI-A-binding proteins). The evolutionarily conserved NAB proteins, NAB1 and NAB2 are corepressors of EGF-1/NGFI-A. Both NAB1 and NAB2 contain an amino terminal NAB conserved domain 1 (NCB1), which is required for binding NGFI-A, and a carboxy terminal NCD2 domain, which is responsible for the repressor function of NAB proteins. NAB2 is principally localized in the nucleus and may play a role in the downregulation of NGFI-A activity as well as in controlling fundamental processes such as cell division, differentiation and apoptosis. NAB2 localizes to chromosome 12q13.3-14.1, a region that is rearranged in several solid tumors, lipomas, and liposarcomas. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is NAB2 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-11299R-BF680) validated for, and at what dilution?
bs-11299R-BF680 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NAB2 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-11299R-BF680) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-11299R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NAB2 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-11299R-BF680)?
KLH conjugated synthetic peptide derived from human NAB2
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-11299R-BF680)?
AbBy Fluor-680 on bs-11299R-BF680: excitation / emission 679nm/702nm.