phospho-IL2 Receptor beta (Tyr364) Polyclonal Antibody, Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-11300R-Cy5-100uL |
| Product Name | phospho-IL2 Receptor beta (Tyr364) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Sheep, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human IL2 Receptor beta around the phosphorylation site of Tyr364 |
| Modification Site | Tyr364 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3560 |
| Subcellular location | Cell membrane |
| Synonyms | Interleukin-2 receptor subunit beta; IL-2 receptor subunit beta; IL-2R subunit beta; IL-2RB; High affinity IL-2 receptor subunit beta; p70-75; p75; IL2RB |
| Background | IL2 Receptor beta (CD122) is a member of the immunoglobulin superfamily that forms the high affinity IL2 receptor with CD25 and CD132. This receptor chain, which is also shared by the IL15 receptor, is constitutively expressed by NK cells and at lower levels by T cells, B cells, monocytes, and macrophages. The IL2 Receptor beta chain can combine with either the common gamma subunit (gamma c) alone or the gamma c subunit and the IL2 Receptor alpha subunit to generate intermediate or high affinity IL2 receptor complexes, respectively. CD122 levels can be upregulated by activation stimuli such as IL2. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-IL2 Receptor beta (Tyr364) Polyclonal Antibody, Cy5 Conjugated (bs-11300R-Cy5) validated for, and at what dilution?
bs-11300R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-11300R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-IL2 Receptor beta (Tyr364) Polyclonal Antibody, Cy5 Conjugated (bs-11300R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human IL2 Receptor beta around the phosphorylation site of Tyr364
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-11300R-Cy5)?
Cy5 on bs-11300R-Cy5: excitation / emission 625,650nm/670nm.