NOVA1 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11324R-BF350-100uL |
| Product Name | NOVA1 Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NOVA1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Swiss Prot | P51513 |
| Subcellular location | Nucleus |
| Synonyms | Neuro oncological ventral antigen 1; Neuro-oncological ventral antigen 1; NOVA 1; Nova-1; Nova1; NOVA1_HUMAN; Onconeural ventral antigen 1; Paraneoplastic Ri antigen; RNA binding protein Nova 1; RNA-binding protein Nova-1; Ventral neuron specic protein 1; Ventral neuron-specic protein 1. |
| Background | Nova-1 and Nova-2 are members of a superfamily of protein regulators of RNA metabolism in neurons. Both are nuclear RNA binding proteins with K homology motifs, conserved protein sequences which bind to RNA (1,2). Nova proteins, normally sequestered in the central nervous system, are expressed by systemic tumors in patients with the autoimmune disorder paraneoplastic opsoclonus-myoclonus ataxia (POMA) (3,4). Nova-1 is expressed in the hindbrain and ventral spinal cord and Nova-2 is expressed in the neocortex and hippocampus (4). Nova-1 is necessary for regulating neuron-specific alternative splicing of the glycine receptor Alpha2 pre-mRNA (5). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NOVA1 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-11324R-BF350) validated for, and at what dilution?
bs-11324R-BF350 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NOVA1 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-11324R-BF350) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Chicken.
How should bs-11324R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NOVA1 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-11324R-BF350)?
KLH conjugated synthetic peptide derived from human NOVA1
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-11324R-BF350)?
AbBy Fluor-350 on bs-11324R-BF350: excitation / emission 346nm/442nm.