CPEB3 Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-11340R-PerCP-Cy5.5-100uL |
| Product Name | CPEB3 Polyclonal Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CPEB3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | CPE-binding protein 3; CPE-BP3; CPEB 3; Cpeb3; CPEB3_HUMAN; Cytoplasmic polyadenylation element binding protein 3; Cytoplasmic polyadenylation element-binding protein 3; hCPEB-3; KIAA0940. |
| Background | The regulated translation of messenger RNA is essential for cell-cycle progression, establishment of the body plan during early development and modulation of key activities in the central nervous system. Cytoplasmic polyadenylation, one mechanism of controlling translation, is driven by cytoplasmic polyadenylation element binding proteins, called CPEBs. CPEB3 (cytoplasmic polyadenylation element binding protein 3) is a 698 amino acid protein that contains two RNA recognition motif (RRM) domains and, like other CPEB proteins, may play a role in the maturation of the central nervous system. CPEB3 exists as multiple alternatively spliced isoforms that are encoded by a gene which maps to human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CPEB3 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-11340R-PERCP-CY5-5) validated for, and at what dilution?
BS-11340R-PERCP-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should BS-11340R-PERCP-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CPEB3 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-11340R-PERCP-CY5-5)?
KLH conjugated synthetic peptide derived from human CPEB3
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BS-11340R-PERCP-CY5-5)?
PerCP-Cy5.5 on BS-11340R-PERCP-CY5-5: excitation / emission 482nm/694nm.
