Hippocalcin Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11348R-RBITC-100uL |
| Product Name | Hippocalcin Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Cow, Chicken, Rabbit |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Hippocalcin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | BDR 2; BDR2; Calcium binding protein BDR 2; Calcium binding protein BDR2; Calcium-binding protein BDR-2; Hpca; HPCA_HUMAN; Neuron specic calcium binding protein hippocalcin; Neuron specic calcium-binding protein hippocalcin; Neuron-specic calcium-binding protein hippocalcin;P23K. |
| Background | Hippocalcin is a neuron-specific calcium-binding protein found primarily in the plasma membrane of brain and retinal tissue, with increased expression observed in hippocampal pyramidal cells. Through its calcium-dependent signal regulation, hippocalcin can both inhibit rhodopsin kinase and increase phospholipase D2 expression. In order to regulate kinase and phospholipase activity, hippocalcin must bind to the plasma membrane where it can then bind two calcium ions for use in signal regulation. The hippocalcin protein is highly conserved in mouse, rat and human tissue and has a suggested role in neural plasticity and associative memory by contributing to the survival of neurons during aging. The loss of hippocalcin expression is thought to contribute to age-related impairment of post-synaptic functions related to neuronal degradation. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Hippocalcin Polyclonal Antibody, RBITC Conjugated (bs-11348R-RBITC) validated for, and at what dilution?
bs-11348R-RBITC is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Hippocalcin Polyclonal Antibody, RBITC Conjugated (bs-11348R-RBITC) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Chicken, Rabbit.
How should bs-11348R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Hippocalcin Polyclonal Antibody, RBITC Conjugated (bs-11348R-RBITC)?
KLH conjugated synthetic peptide derived from human Hippocalcin
What are the excitation and emission wavelengths of the RBITC conjugate (bs-11348R-RBITC)?
RBITC on bs-11348R-RBITC: excitation / emission 540nm/625nm.