RIMS3 Polyclonal Antibody, Biotin Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-11359R-Biotin-100uL |
| Product Name | RIMS3 Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Horse, Chicken, Rabbit, Zebrafish |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RIMS3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 9783 |
| Subcellular location | Cell membrane |
| Synonyms | NIM 3; NIM3; Rab 3 interacting molecule 3; Regulating synaptic membrane exocytosis 3; Regulating synaptic membrane exocytosis protein 3; RIMS 3; RIMS-3; RIM 3; RIM3; RIM3 gamma; RIMS3; RIMS3_HUMAN. |
| Background | Rab 3, a neural/neuroendocrine-specific member of the Rab family, is involved in Ca2+-regulated exocytosis and functions in an inhibitory capacity, controlling the recruitment and pooling of secretory vesicles at the plasma membrane. The Rim (Rab 3 interacting molecule) family of proteins (Rim1, Rim2, Rim3 and Rim4) are multidomain adaptors that regulate Rab 3 activity and sub-sequent neurotransmitter release. Rim3, also known as RIMS3 (regulating synaptic membrane exocytosis 3) or NIM3, is a 308 amino acid member of the Rim family. Localized to the synapse and to cell junctions, Rim3 contains one C2 domain and is thought to play an important role in the regulation of synaptic membrane exocytosis. Rim3, a protein that may be phosphorylated upon DNA damage, is expressed throughout the body with highest levels present in brain tissue. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is RIMS3 Polyclonal Antibody, Biotin Conjugated (bs-11359R-Biotin) validated for, and at what dilution?
bs-11359R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-11359R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RIMS3 Polyclonal Antibody, Biotin Conjugated (bs-11359R-Biotin)?
KLH conjugated synthetic peptide derived from human RIMS3