SRC Polyclonal Antibody, Cy3 Conjugated
Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.", "Category"=>"Conjugated Primary Antibody", "Clonality"=>"Polyclonal", "Concentration"=>"1ug/ul", "Conjugation"=>"Cy3", "Cross Reactive Species"=>"Human, Mouse, Rat", "Datasheet URL"=>"https://www.biossusa.com/store/datasheets/bs-1135R-Cy3", "Excitation Emission"=>"512,550nm/570,615nm", "Gene ID"=>"6714", "Gene ID Link"=>"Entrez Gene: 6714", "Host"=>"Rabbit", "Immunogen Sequence"=>"2-100/541", "Isotype"=>"IgG", "Lead Time"=>"10 to 15 business days.", "Modification"=>"Unmodified", "Predicted Reactivity"=>"Dog, Cow", "Product URL"=>"https://www.biossusa.com/products/bs-1135R-Cy3", "Purification"=>"Purified by Protein A.", "Size"=>"100ul", "Source"=>"KLH conjugated synthetic peptide derived from human SRC", "Storage"=>"Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.", "Storage Buffer"=>"Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.", "Storage Condition"=>"Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.", "Subcellular Locations"=>"Cytoplasm, Nucleus, Cell membrane", "Swiss Prot"=>"P12931", "Swiss Prot Link"=>"Swiss Prot: P12931", "Synonyms"=>"ASV; Avian sarcoma virus; c SRC; CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA; cSrc; EC 2.7.10.2; Neuronal CSRC tyrosine specic protein kinase; Neuronal SRC; Oncogene SRC; OTTHUMP00000030931; OTTHUMP00000174476; OTTHUMP00000174477; p60 Src; p60-Src; p60Src; pp60c src; pp60c-src; pp60csrc; Proto oncogene tyrosine protein kinase Src; Proto-oncogene c-Src; Proto-oncogene tyrosine-protein kinase Src; Protooncogene SRC; protooncogene SRC Rous sarcoma; Src; SRC Oncogene; SRC_MOUSE; SRC1; Tyrosine kinase pp60c src; Tyrosine protein kinase SRC 1; Tyrosine protein kinase SRC1; v src avian sarcoma Schmidt Ruppin A2 viral oncogene homolog; V src sarcoma Schmidt Ruppin A 2 viral oncogene homolog avian; v src sarcoma Schmidt Ruppin A 2 viral oncogene homolog avian; vsrc avian sarcoma Schmidt Ruppin A2 viral oncogene homolog; SRC_HUMAN.", "Target Protein"=>"SRC"} -->
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1135R-Cy3 |
| Product Name | SRC Polyclonal Antibody, Cy3 Conjugated |
| Applications | WB, IF(IHC-P), IF(IHC-F), IF(ICC) |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SRC |
| Immunogen Range | 2-100/541 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6714 |
| Swiss Prot | P12931 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | ASV; Avian sarcoma virus; c SRC; CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA; cSrc; EC 2.7.10.2; Neuronal CSRC tyrosine specic protein kinase; Neuronal SRC; Oncogene SRC; OTTHUMP00000030931; OTTHUMP00000174476; OTTHUMP00000174477; p60 Src; p60-Src; p60Src; pp60c src; pp60c-src; pp60csrc; Proto oncogene tyrosine protein kinase Src; Proto-oncogene c-Src; Proto-oncogene tyrosine-protein kinase Src; Protooncogene SRC; protooncogene SRC Rous sarcoma; Src; SRC Oncogene; SRC_MOUSE; SRC1; Tyrosine kinase pp60c src; Tyrosine protein kinase SRC 1; Tyrosine protein kinase SRC1; v src avian sarcoma Schmidt Ruppin A2 viral oncogene homolog; V src sarcoma Schmidt Ruppin A 2 viral oncogene homolog avian; v src sarcoma Schmidt Ruppin A 2 viral oncogene homolog avian; vsrc avian sarcoma Schmidt Ruppin A2 viral oncogene homolog; SRC_HUMAN. |
| Background | c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr --> Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
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