A4GNT Polyclonal Antibody
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-11382R |
| Product Name | A4GNT Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Chicken |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human A4GNT |
| Immunogen Range | 131-180/340 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 51146 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | 4-N-acetylglucosaminyltransferase; A4GCT_HUMAN; A4GNT; Alpha 1 4 N acetylglucosaminyltransferase; Alpha-1; Alpha4GnT; EC 2.4.1.; MGC149493. |
| Background | Alpha 1,4-N-acetylflucosaminyltransferase (Alpha4Gn-T) mediates the biosynthesis of mucin type glycoprotein (O-glycan). Alpha4Gn-T acts as the key enzyme for the formation of the unique glycan GlcNAcalpha1-4Galbeta1-R, and most efficiently transfers N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. Alpha4Gn-T is a single-pass type II membrane protein associated with the Golgi apparatus and contains the conserved DXD motif involved in catalytic activity. It is expressed in stomach and pancreas, as well as in gastric cancer cells. Alpha4Gn-T is not expressed in peripheral blood cells, making it a useful biomarker for pancreatic cancer. Alpha4Gn-T and Mucin 6 expression is upregulated in the gastric mucosa of H.pylori infected patients, which suggest the involvement of ?Gn-T in defense against H. pylori infection. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |