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GCX1/C20orf100 Polyclonal Antibody

Applications

  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)
  • dot-blot

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-11402R-100uL
Product Name GCX1/C20orf100 Polyclonal Antibody
Applications
ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC) DOT-BLOT
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human GCX1/C20orf100
Immunogen Range 201-300/488
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 84969
Subcellular location Nucleus
Synonyms Chromosome 20 open reading frame 100; Chromosome 20 open reading frame 100; dJ1108D11.2; dJ495O3.1; GCX 1; GCX1; GCX-1; Granulosa cell HMG box protein 1; MGC15880; Weakly similar to NONHISTONE CHROMOSOMAL PROTEIN 6A [S.cerevisiae]; Weakly similar to NONHISTONE CHROMOSOMAL PROTEIN 6A [S.cerevisiae]; TOX2_HUMAN.
Background GCX-1 is a 488 amino acid transcription activator that is restricted to expression in the hypothalamus, pituitary, ovary, testis and uterus.Human reproduction is controlled by the hypothalamic-pituitary gonadal axis (HPGA) that is laid down early in fetal development. This expression pattern in the HPGA suggests that this nuclear protein is likely related to specific events in reproduction, particularly in the female. GCX-1 contains a HMG-box domain, which is commonly found in proteins that function as intercellular regulators and transcriptional co-regulators, and are found to be involved in important events such as sex determination and in the regulation of T cell differentiation. There are two isoforms of GCX-1 which are produced as a result of alternative splicing events.
Application Dilution
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
dot-blot

Frequently asked questions

Which applications is GCX1/C20orf100 Polyclonal Antibody (bs-11402R) validated for, and at what dilution?

bs-11402R is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); dot-blot; ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does GCX1/C20orf100 Polyclonal Antibody (bs-11402R) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit.

How should bs-11402R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GCX1/C20orf100 Polyclonal Antibody (bs-11402R)?

KLH conjugated synthetic peptide derived from human GCX1/C20orf100 Immunogen range: 201-300/488.

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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