GNRHR2 Polyclonal Antibody, RBITC Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-11403R-RBITC-100uL |
| Product Name | GNRHR2 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Pig |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GNRHR2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cell membrane |
| Synonyms | GnRH IIR; Putative gonadotropin-releasing hormone II receptor; gonadotropin-releasing hormone type 2 receptor 2; GnRH-II-R; Type II GnRH receptor; GNRR2_HUMAN. |
| Background | Gonadotropin-releasing hormone (GnRH) is released in a pulsatile manner that varies with the reproductive cycle. This hypothalamic hormone is transported to the pituitary, where it binds to specific receptors and regulates the synthesis and release of luteinizing hormone (LH) and follicle-stimulating hormone (FSH). Localized to the cell membrane, GnRHR2 mediates its own receptor activity via association with G proteins, thereby activating a phosphatidylinositol-calcium second messenger system that regulates GnRHR2 function. GnRHR2 is thought to have potent antiproliferative effects on ovarian and endometrial cancer cells, suggesting a possible role in tumor suppression. Due to alternative splicing events, GnRHR2 is expressed as two isoforms. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GNRHR2 Polyclonal Antibody, RBITC Conjugated (bs-11403R-RBITC) validated for, and at what dilution?
bs-11403R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-11403R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GNRHR2 Polyclonal Antibody, RBITC Conjugated (bs-11403R-RBITC)?
KLH conjugated synthetic peptide derived from human GNRHR2
What are the excitation and emission wavelengths of the RBITC conjugate (bs-11403R-RBITC)?
RBITC on bs-11403R-RBITC: excitation / emission 540nm/625nm.