HSD17B8 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11407R-BF647 |
| Product Name | HSD17B8 Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HSD17B8 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7923 |
| Subcellular location | Cytoplasm |
| Synonyms | 17 beta HSD 8; 17 beta hydroxysteroid dehydrogenase 8; 17-beta-HSD 8; 17-beta-hydroxysteroid dehydrogenase 8; 3-oxoacyl-[acyl-carrier-protein] reductase; Beta ketoacyl [acyl carrier protein] reductase like; D6S2245E; DHB8_HUMAN; dJ1033B10.9; Estradiol 17 beta dehydrogenase 8; Estradiol 17-beta-dehydrogenase 8; Estrogen 17 oxidoreductase; FABG; FABGL; H2 KE6; HKE6; HSD17B8; Hydroxysteroid 17 beta dehydrogenase 8; 17beta hydroxysteroid dehydrogenase type 8; Ke-6; KE6; Protein Ke6; Really interesting new gene 2 protein; RING2; SDR30C1; Short chain dehydrogenase/reductase family 30C member 1; Testosterone 17 beta dehydrogenase 8; Testosterone 17-beta-dehydrogenase 8. |
| Background | 17beta-HSD8 belongs to the 17beta-HSD family of proteins that regulate the availability of steroids within a tissue. 17beta-HSD8 converts active steroids to their inactive form through its oxidative activity. It is a key player in the inactivation of Estradiol and Testosterone. 17beta-HSD8 is predominantly expressed in placenta, endometrium and prostate but can also be found in liver, and pancreas, with lowest levels found in testis, ovary and kidney. It has been proposed that a reduction in the levels of 17beta-HSD8 may lead to abnormal elevations in the local level of sex steroids, which can lead to recessive renal cystic disease. It has also been suggested that low levels of 17beta-HSD proteins may result in an underdeveloped urogenital system. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is HSD17B8 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-11407R-BF647) validated for, and at what dilution?
bs-11407R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HSD17B8 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-11407R-BF647) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Rabbit.
How should bs-11407R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HSD17B8 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-11407R-BF647)?
KLH conjugated synthetic peptide derived from human HSD17B8
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-11407R-BF647)?
AbBy Fluor-647 on bs-11407R-BF647: excitation / emission 650nm/665nm.