NMUR1/GPR66 Polyclonal Antibody

$RSC96 cells probed with NMUR1\/GPR66 Polyclonal Antibody, Unconjugated (bs-11420R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-FITC) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).$

RSC96 cells probed with NMUR1\/GPR66 Polyclonal Antibody, Unconjugated (bs-11420R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-FITC) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

$A549 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with NMUR1\/GPR66 Polyclonal Antibody (bs-11420R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).$

A549 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with NMUR1\/GPR66 Polyclonal Antibody (bs-11420R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Applications

ELISA
FCM
IHC-P

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Cow
Sheep
Pig
Horse

Overview
Catalog #	bs-11420R
Product Name	NMUR1/GPR66 Polyclonal Antibody
Applications	ELISA, FCM, IHC-P
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Cow, Sheep, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human NMUR1
Immunogen Range	31-80/426
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10316
Swiss Prot	Q9HB89
Subcellular location	Cell membrane
Synonyms	FM-3; G protein-coupled receptor 66; G-protein coupled receptor 66; G-protein coupled receptor FM-3; GPR66; Growth hormone secretagogue receptor family Member 3; Neuromedin U Receptor 1; Neuromedin-U receptor 1; NMU-R1; NMUR1; NMUR1_HUMAN.
Background	Neuromedin U is a neuropeptide with high activity on smooth muscle. It is widely expressed in gastrointestinal systems and central nervous system (CNS). Peripheral activities of neuromedin U include smooth muscle stimulation, ion transport alterations in the gut and the regulation of local blood flow and adrenocortical function. Neuromedin U receptors 1 and 2 (NMUR1 and NMUR2) are multi-pass membrane proteins that belong to the G-protein coupled receptor 1 family of proteins. Both NMUR1 and NMUR2 act as receptors for the neuromedin U neuropeptide. NMUR1 is detected in peripheral organs, particularly in urogenital and gastrointestinal systems, with highest levels in testis. It?s expression in CNS is low, but the protein has been detected in cerebellum, hippocampus, dorsal root ganglia and spinal cord. NMUR2 is predominantly detected in central nervous system with highest levels detected in medulla oblongata, spinal cord and thalamus. It may also be detected in testis but has low levels of expression in peripheral tissues.
Application Dilution
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400

Applications

Reactivity

Predicted Reactivity

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