Neuropeptide S Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-11422R-BF488 |
| Product Name | Neuropeptide S Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Neuropeptide S |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 594857 |
| Subcellular location | Secreted |
| Synonyms | NeuropeptideS; Neuropeptide-S; NPS; NPS_HUMAN. |
| Background | Neuropeptides are regulators of synaptic transmission and their effects are mediated by G-protein coupled receptors. NPS (Neuropeptide S) is a 20 amino acid peptide cleaved from a larger precursor that contains a hydrophobic signal peptide and proteolytic cleavage processing sites. The N-terminal residue of NPS is always a serine regardless of the species. NPS is predominantly found in the central nervous system and plays an important role regulating sleep/wake functions, locomotion, arousal/anxiety responses and food intake. NPS functions by binding and activating its receptor, NPSR, and increasing intracellular calcium levels thereby acting as an excitatory transmitter. In addition, NPS stimulates the hypothalamo-pituitary adrenal (HPA) axis via the release of corticotropin-releasing factor (CRF) and arginine vasopressin (AVP). NPS and its receptor NPSR may also play a role in asthma pathogenesis. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Neuropeptide S Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-11422R-BF488) validated for, and at what dilution?
bs-11422R-BF488 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-11422R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Neuropeptide S Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-11422R-BF488)?
KLH conjugated synthetic peptide derived from human Neuropeptide S
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-11422R-BF488)?
AbBy Fluor-488 on bs-11422R-BF488: excitation / emission 499nm/519nm.