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NPSR1 Polyclonal Antibody, RBITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
Overview
Catalog # bs-11430R-RBITC-100uL
Product Name NPSR1 Polyclonal Antibody, RBITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NPSR1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 387129
Swiss Prot Q6W5P4
Subcellular location Cytoplasm, Cell membrane
Synonyms ASRT2; G protein coupled receptor 154; G protein coupled receptor for asthma susceptibility; G protein coupled receptor PGR14; GPR 154; gpr154; GPRA; MVTR; Neuropeptide S receptor 1; Neuropeptide S receptor; nps receptor; NPSR 1; NPSR; NPSR1; PGR 14; PGR14; PGR-14; Vasopressin receptor related receptor 1; VRR 1; VRR1; NPSR1_HUMAN.
Background G protein-coupled receptors (GPRs), also known as seven transmembrane receptors, heptahelical receptors or 7TM receptors, comprise a superfamily of proteins that play a role in many different stimulus-response pathways. G protein coupled receptors translate extracellular signals into intracellular signals (G protein activation) and they respond to a variety of signaling molecules, such as hormones and neurotransmitters. GPR154 (G-protein coupled receptor 154), also known as NPSR1 (neuropeptide S receptor), GPRA (G-protein coupled receptor for asthma susceptibility) or PGR14, is a 371 amino acid protein that is thought to play a role in autocrine or paracrine signaling pathways. Ubiquitously expressed, GPR154 exists as nine alternatively spliced isoforms. Defects in the gene encoding GPR154 is the cause of asthma-related traits type 2 (ASRT2).
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is NPSR1 Polyclonal Antibody, RBITC Conjugated (bs-11430R-RBITC) validated for, and at what dilution?

bs-11430R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-11430R-RBITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NPSR1 Polyclonal Antibody, RBITC Conjugated (bs-11430R-RBITC)?

KLH conjugated synthetic peptide derived from human NPSR1

What are the excitation and emission wavelengths of the RBITC conjugate (bs-11430R-RBITC)?

RBITC on bs-11430R-RBITC: excitation / emission 540nm/625nm.

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