TRAP80 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11437R-Cy5.5-100uL |
| Product Name | TRAP80 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CRSP77 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9440 |
| Swiss Prot | Q9NVC6 |
| Subcellular location | Nucleus |
| Synonyms | CRSP6; CRSP77; DRIP80; TRAP80; Mediator of RNA polymerase II transcription subunit 17; Activator-recruited cofactor 77 kDa component; ARC77; Cofactor required for Sp1 transcriptional activation subunit 6; CRSP complex subunit 6; Mediator complex subunit 17; Thyroid hormone receptor-associated protein complex 80 kDa component; Transcriptional coactivator CRSP77; Vitamin D3 receptor-interacting protein complex 80 kDa component; MED17; DRIP77 |
| Background | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is TRAP80 Polyclonal Antibody, Cy5.5 Conjugated (BS-11437R-CY5-5) validated for, and at what dilution?
BS-11437R-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TRAP80 Polyclonal Antibody, Cy5.5 Conjugated (BS-11437R-CY5-5) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.
How should BS-11437R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TRAP80 Polyclonal Antibody, Cy5.5 Conjugated (BS-11437R-CY5-5)?
KLH conjugated synthetic peptide derived from human CRSP77
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-11437R-CY5-5)?
Cy5.5 on BS-11437R-CY5-5: excitation / emission 675nm/694nm.