THRB1 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11440R-BF488-100uL |
| Product Name | THRB1 Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Sheep, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Thyroid Hormone Receptor beta |
| Immunogen Range | 201-300/461 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7068 |
| Swiss Prot | P10828 |
| Subcellular location | Nucleus |
| Synonyms | Thyroid Hormone Receptor beta; Avian erythroblastic leukemia viral v erb a oncogene homolog 2; C ERBA 2; C ERBA BETA; c-erbA-2; c-erbA-beta; ERBA 2; ERBA BETA; ERBA2; Erythroblastic leukemia viral v erb a oncogene homolog 2 avian; generalized resistance to thyroid hormone; GRTH; MGC126109; MGC126110; NR1A2; Nuclear receptor subfamily 1 group A member 2; Oncogene ERBA2; PRTH; THB_HUMAN; THR 1; THR1; THRB 1; |
| Background | Thyroid hormone receptors (TRs) are ligand-dependent transcription factors that mediate the biological activities of thyroid hormone (T3). Thyroid hormone receptor b2 (TRb2) is a high affinity receptor for triiodothyronine which belongs to the nuclear hormone receptor family and the NR1 subfamily. It is composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain. Defects in the receptor result in generalized thyroid hormone resistance (GTHR). GTHR is transmitted as an autosomal dominant trait, but an autosomal recessive form also exists. The disease is characterized by goiter, abnormal mental functions, increased susceptibility to infections, abnormal growth and bone maturation, tachycardia and deafness. GTHR patients also have high levels of circulating thyroid hormones (T3-T4), with normal or slightly elevated thyroid stimulating hormone. |
| Application Dilution | |
| WB | 1:50-200 |
| FCM | 1:500-1000 |
| IF(IHC-P) | 1:100-500 |
| IF(IHC-F) | 1:200-400 |
| IF(ICC) | 1:20-100 |
Frequently asked questions
Which applications is THRB1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-11440R-BF488) validated for, and at what dilution?
bs-11440R-BF488 is validated for WB (1:50-200); FCM (1:500-1000); IF(IHC-P) (1:100-500); IF(IHC-F) (1:200-400); IF(ICC) (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does THRB1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-11440R-BF488) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep, Chicken, Rabbit.
How should bs-11440R-BF488 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise THRB1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-11440R-BF488)?
KLH conjugated synthetic peptide derived from human Thyroid Hormone Receptor beta Immunogen range: 201-300/461.
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-11440R-BF488)?
AbBy Fluor-488 on bs-11440R-BF488: excitation / emission 499nm/519nm.