BCAS1 Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-11462R-BF750-100uL |
| Product Name | BCAS1 Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human BCAS1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm |
| Synonyms | AIBC 1; AIBC1; Amplied and overexpressed in breast cancer; BCAS 1; BCAS1; BCAS1_HUMAN; Breast carcinoma amplied sequence 1; Breast carcinoma-amplied sequence 1; Novel amplied in breast cancer 1. |
| Background | NaBC1 is a protein found amplified in most breast carcinoma forms. It is expressed primarily as a cytoplasmic, detergent-stable homodimer that has a tendency to interact with DYNLL1 (PIN) and DYNLL2. Breast tumor lines that exhibit 20q13.2 gene amplification express much higher levels of the protein as compared to the levels found in other breast cancer lines that do not overexpress the NaBC1 mRNA. However, this upregulation does not affect growth rate or anchoring abilities of a cell, indicating the oncogenic properties of NaBC1 differ from that of other oncogenes. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is BCAS1 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-11462R-BF750) validated for, and at what dilution?
bs-11462R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-11462R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise BCAS1 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-11462R-BF750)?
KLH conjugated synthetic peptide derived from human BCAS1
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-11462R-BF750)?
AbBy Fluor-750 on bs-11462R-BF750: excitation / emission 749nm/775nm.