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BCAS1 Polyclonal Antibody, PerCP Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-11462R-PerCP-100uL
Product Name BCAS1 Polyclonal Antibody, PerCP Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human BCAS1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Cytoplasm
Synonyms AIBC 1; AIBC1; Amplied and overexpressed in breast cancer; BCAS 1; BCAS1; BCAS1_HUMAN; Breast carcinoma amplied sequence 1; Breast carcinoma-amplied sequence 1; Novel amplied in breast cancer 1.
Background NaBC1 is a protein found amplified in most breast carcinoma forms. It is expressed primarily as a cytoplasmic, detergent-stable homodimer that has a tendency to interact with DYNLL1 (PIN) and DYNLL2. Breast tumor lines that exhibit 20q13.2 gene amplification express much higher levels of the protein as compared to the levels found in other breast cancer lines that do not overexpress the NaBC1 mRNA. However, this upregulation does not affect growth rate or anchoring abilities of a cell, indicating the oncogenic properties of NaBC1 differ from that of other oncogenes.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is BCAS1 Polyclonal Antibody, PerCP Conjugated (bs-11462R-PerCP) validated for, and at what dilution?

bs-11462R-PerCP is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-11462R-PerCP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise BCAS1 Polyclonal Antibody, PerCP Conjugated (bs-11462R-PerCP)?

KLH conjugated synthetic peptide derived from human BCAS1

What are the excitation and emission wavelengths of the PerCP conjugate (bs-11462R-PerCP)?

PerCP on bs-11462R-PerCP: excitation / emission 482nm/677nm.

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