SEMA4F Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-11478R-BF555-100uL |
| Product Name | SEMA4F Polyclonal Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SEMA4F |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10505 |
| Swiss Prot | O95754 |
| Subcellular location | Cell membrane |
| Synonyms | S4F; SEMAM; SEMAW; M-SEMA; PRO2353; m-Sema-M; Semaphorin-4F; Semaphorin-M; Sema M; Semaphorin-W; Sema W; SEMA4F |
| Background | Has growth cone collapse activity against retinal ganglion-cell axons. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is SEMA4F Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-11478R-BF555) validated for, and at what dilution?
bs-11478R-BF555 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-11478R-BF555 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SEMA4F Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-11478R-BF555)?
KLH conjugated synthetic peptide derived from human SEMA4F
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-11478R-BF555)?
AbBy Fluor-555 on bs-11478R-BF555: excitation / emission 553nm/568nm.