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SEMA4F Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Cow
  • Horse
Overview
Catalog # bs-11478R-PE-100uL
Product Name SEMA4F Polyclonal Antibody, PE Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat, Dog, Cow, Horse
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SEMA4F
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 10505
Swiss Prot O95754
Subcellular location Cell membrane
Synonyms S4F; SEMAM; SEMAW; M-SEMA; PRO2353; m-Sema-M; Semaphorin-4F; Semaphorin-M; Sema M; Semaphorin-W; Sema W; SEMA4F
Background Has growth cone collapse activity against retinal ganglion-cell axons.
Application Dilution
WB 1:300-5000
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Frequently asked questions

Which applications is SEMA4F Polyclonal Antibody, PE Conjugated (bs-11478R-PE) validated for, and at what dilution?

bs-11478R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-11478R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SEMA4F Polyclonal Antibody, PE Conjugated (bs-11478R-PE)?

KLH conjugated synthetic peptide derived from human SEMA4F

What are the excitation and emission wavelengths of the PE conjugate (bs-11478R-PE)?

PE on bs-11478R-PE: excitation / emission 496,564nm/578nm.