SPAG16 Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11485R-Biotin-100uL |
| Product Name | SPAG16 Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Sheep, Horse, Rabbit |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SPAG16 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 79582 |
| Swiss Prot | Q8N0X2 |
| Subcellular location | Cytoplasm |
| Synonyms | PF20; WDR29; Sperm-associated antigen 16 protein; Pf20 protein homolog; SPAG16 |
| Background | Necessary for sperm flagellar function. Plays a role in motile ciliogenesis. May help to recruit STK36 to the cilium or apical surface of the cell to initiate subsequent steps of construction of the central pair apparatus of motile cilia (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is SPAG16 Polyclonal Antibody, Biotin Conjugated (bs-11485R-Biotin) validated for, and at what dilution?
bs-11485R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SPAG16 Polyclonal Antibody, Biotin Conjugated (bs-11485R-Biotin) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Horse, Rabbit.
How should bs-11485R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SPAG16 Polyclonal Antibody, Biotin Conjugated (bs-11485R-Biotin)?
KLH conjugated synthetic peptide derived from human SPAG16