VAX2 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-11497R-BF680-100uL |
| Product Name | VAX2 Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Sheep, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human VAX2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Nucleus |
| Synonyms | DRES93; Ventral anterior homeobox 2; VAX2_HUMAN. |
| Background | VAX2 is a 290 amino acid homeobox protein that plays a crucial role in development of the eye, particularly in the specification of the ventral optic vesicle and in establishment of a correct dorsoventral pattern. VAX2 acts as a transcription factor with VAX1 to cooperatively regulate retinal differentiation, neuroepithelial cell proliferation and axial polarization in the retina. Together, VAX1 and VAX2 repress transcription of Pax-6, a strong inducer of retinal development. VAX1 and VAX2 localize to the nucleus and contain one homeobox DNA-binding domain each. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is VAX2 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-11497R-BF680) validated for, and at what dilution?
bs-11497R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-11497R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise VAX2 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-11497R-BF680)?
KLH conjugated synthetic peptide derived from human VAX2
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-11497R-BF680)?
AbBy Fluor-680 on bs-11497R-BF680: excitation / emission 679nm/702nm.