GFRA3/GDNF Receptor alpha 3 Antibody, AbBy Fluor-647 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-11501r-bf647 |
| Product Name | GFRA3/GDNF Receptor alpha 3 Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
|
| Predicted Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GFR alpha 3 |
| Immunogen Range | 101-185/400 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cell membrane |
| Synonyms | GDNF family receptor alpha 3; GFR alpha 3; GDNF family receptor alpha-3; GDNF receptor alpha-3; GDNFR-alpha-3; GFR-alpha-3; GFRA3; GFRA3_HUMAN; Glial cell line derived neurotrophic factor receptor alpha 3; GPI linked receptor. |
| Background | Glial cell line-derived neurotrophic factor (GDNF) and the related neurotrophic factor neurturin (NTN) are potent survival factors for central and peripheral neurons. GDNF is a glycosylated, disulfide-bonded homodimer that is distantly related to the TGF Beta superfamily of growth factors. Three receptors for these factors, GFR Alpha-1 (also designated GDNFR-Alpha, RETL1 or TrnR-1), GFR Alpha-2 (also designated GDNFR-Beta, RETL2, NTNR-Alpha or TrnR-2) and GFR Alpha-3 have been identified. The receptors do not contain transmembrane domains and are attached to the cell membrane by glycosyl-phosphoinositol linkage. Both GFR Alpha-1 and GFR Alpha-2 have been shown to mediate the GDNF-dependent and NTN-dependent phosphorylation and activation of the tyrosine kinase Ret. GFR Alpha-3 is expressed only during development. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is GFRA3/GDNF Receptor alpha 3 Antibody, AbBy Fluor-647 Conjugated (bs-11501R-BF647) validated for, and at what dilution?
bs-11501R-BF647 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-11501R-BF647 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GFRA3/GDNF Receptor alpha 3 Antibody, AbBy Fluor-647 Conjugated (bs-11501R-BF647)?
KLH conjugated synthetic peptide derived from human GFR alpha 3 Immunogen range: 101-185/400.
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-11501R-BF647)?
AbBy Fluor-647 on bs-11501R-BF647: excitation / emission 650nm/665nm.