BBS9 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with BBS9 Polyclonal Antibody, Unconjugated (bs-11511R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with BBS9 Polyclonal Antibody, Unconjugated (bs-11511R) at 1:400 overnight at 4\u00b0C, DAB staining.

Lane 1: Mouse Uterus tissue lysates; Lane 2: Rat Testis tissue lysates; Lane 3: Human Huvec cell lysates; Lane 4: Human U87MG cell lysates ; Lane 5: Human Hela cell lysates ; Lane 6: Human 293T cell lysates probed with BBS9 Polyclonal Antibody, Unconjugated (bs-11511R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Predicted Reactivity

Human
Mouse
Dog
Sheep
Pig
Horse
Rabbit

Overview
Catalog #	bs-11511R
Product Name	BBS9 Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Rat
Predicted Reactivity	Human, Mouse, Dog, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human BBS9
Immunogen Range	244-320/887
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	27241
Swiss Prot	Q3SYG4
Subcellular location	Cytoplasm, Cell membrane
Synonyms	B1; D1; C18; PTHB1; Protein PTHB1; Bardet-Biedl syndrome 9 protein; Parathyroid hormone-responsive B1 gene protein; BBS9
Background	The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. Required for proper BBSome complex assembly and its ciliary localization.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500

Applications

Reactivity

Predicted Reactivity

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