FADS2 Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11516R-PerCP-Cy5.5 |
| Product Name | FADS2 Polyclonal Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
WB
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human FADS2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm |
| Synonyms | D6D; Delta6 desaturase; Delta6 fatty acid desaturase; DES 6; DES6; FADS 2; FADSD 6; FADSD6; Fatty acid desaturase 2; linoleoyl-CoA desaturase delta-6-desaturase like 2; LLCDL 2; LLCDL2; SLL0262; TU 13; TU13; FADS2_HUMAN. |
| Background | Members of the fatty acid desaturase (FADS) family, including FADS1, FADS2 and FADS3, regulate the desaturation of fatty acids by introducing double bonds between defined carbons of fatty acyl chains, thereby playing an essential role in the lipid metabolic pathway. Members of this family share N-terminal cytochrome b5-like domains, C-terminal multiple membrane-spanning desaturase regions and 3 histidine box motifs. FADS2 (fatty acid desaturase 2), also known as D6D, DES6, LLCDL2 or TU13, is a 444 amino acid multi-pass membrane protein that localizes to the endoplasmic reticulum and contains one cytochrome b5 heme-binding domain. Expressed in adult and fetal heart and in adult liver, brain, lung and retina, FADS2 functions as a component of a lipid metabolic pathway and catalyzes the first step in the pathway, namely the formation of unsaturated fatty acids from polyunsaturated fatty acids. Defects in the gene encoding FADS2 are the cause of cause of fatty acid delta-6-desaturase deficiency, an affliction that is characterized by skin abnormalities, corneal ulceration and growth failure. Multiple isoforms of FADS2 exist due to alternative splicing events. |
| Application Dilution | |
| WB | 1:500-2000 |
Frequently asked questions
Which applications is FADS2 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-11516R-PERCP-CY5-5) validated for, and at what dilution?
BS-11516R-PERCP-CY5-5 is validated for WB (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does FADS2 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-11516R-PERCP-CY5-5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Pig, Horse, Chicken, Rabbit.
How should BS-11516R-PERCP-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise FADS2 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-11516R-PERCP-CY5-5)?
KLH conjugated synthetic peptide derived from human FADS2
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BS-11516R-PERCP-CY5-5)?
PerCP-Cy5.5 on BS-11516R-PERCP-CY5-5: excitation / emission 482nm/694nm.