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FOXD3 Polyclonal Antibody, AbBy Fluor-405 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Chicken
  • Rabbit
Overview
Catalog # bs-11518R-BF405-100uL
Product Name FOXD3 Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Chicken, Rabbit
Specifications
Conjugation AbBy Fluor-405
Host Rabbit
Source KLH conjugated synthetic peptide derived from human FOXD3
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 27022
Swiss Prot Q9UJU5
Subcellular location Nucleus
Synonyms Forkhead box protein D3; HNF3/FH transcription factor genesis; HFH2
Background This gene belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. Mutations in this gene cause autoimmune susceptibility 1. Binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. Also acts as a transcriptional activator. Promotes development of neural crest cells from neural tube progenitors. Restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. Required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is FOXD3 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-11518R-BF405) validated for, and at what dilution?

bs-11518R-BF405 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does FOXD3 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-11518R-BF405) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Chicken, Rabbit.

How should bs-11518R-BF405 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise FOXD3 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-11518R-BF405)?

KLH conjugated synthetic peptide derived from human FOXD3

What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-11518R-BF405)?

AbBy Fluor-405 on bs-11518R-BF405: excitation / emission 401nm/421nm.